Product Overview

LunaGel™ Ultrapure GelMA – Optimized for Immune & Translational Research. LunaGel™ Ultrapure GelMA is a highly purified, photocrosslinkable extracellular matrix (ECM) based on Rousselot X-Pure® GelMA, designed for precise control over matrix porosity and stiffness in 3D cell culture applications.

Endotoxins, particularly lipopolysaccharides (LPS), can trigger inflammatory responses in immune cells, altering their interactions with other cell types and compromising the reliability of therapeutic testing in 3D cell culture models. LunaGel™ Ultrapure GelMA overcomes this issue with ultra-low endotoxin levels (<10 EU/g), making it ideal for macrophage studies, immuno-oncology models, and translational research where immune activation must be tightly controlled.

Viable cells, organoids, and spheroids cultured in gelatin based LunaGel™ photocrosslinkable extracellular matrix (ECMs) can be easily harvested using a LunaGel™ Cell Recovery Kit (SKU0015).

Features

✔ Ready-to-Use Format – Remains liquid above 4°C, eliminating the need for heating or cooling. 

✔ Precise Mechanical Control – Photocrosslinking technology enables tunable stiffness for optimized 3D cell culture conditions. 

✔ High Bioactivity – Supports cell attachment, proliferation, and proteolytic degradation, promoting physiologically relevant cell behaviour. 

✔ Optically Transparent for Imaging
LunaGel™ forms optically clear hydrogels that remain stable at room temperature, ensuring seamless integration with standard imaging systems and bioassays. 

✔ Reliable & Reproducible ECM for 3D Cell Culture
The highly purified gelatin-based LunaGel™ ECM supports the growth and differentiation of viable cells, organoids, and spheroids, providing a consistent and biologically relevant platform for disease modelling, drug screening, and tissue engineering. 

Kit Contents

Each kit provides Everything you need for precise, tunable 3D cell culture: 

Low Stiffness Kits: 

✔ 5 mL LunaGel™ ECM (2x solution, sterile) 

enough LunaGel™ to generate:
✔ 5 vials of freeze-dried photoinitiator (sterile) 

High Stiffness Kits: 

✔ 5 mL LunaGel™ ECM (1.5x solution, sterile) 

✔ 5 vials of freeze-dried photoinitiator (sterile) 

How LunaGel™ Photocrosslinkable Extracellular Matrix​ Works

LunaGel™ is a sterile, ready-to-use ECM solution supplied with a freeze-dried photoinitiator. It is available in two formulations: 

• Low Stiffness Kit: 0 – 6.5 kPa 

• High Stiffness Kit: 0 – 25 kPa 

Simple 3-Step Process: 

1️) Prepare – Reconstitute the photoinitiator in PBS and mix with LunaGel™ ECM and cells.
2️) Crosslink – Use the LunaCrosslinker™ to photocrosslink the solution, forming a stable 3D hydrogel.
3️) Customize Stiffness – Adjust light exposure time to precisely control mechanical properties. 

Simply add your preferred culture media, and you’re ready to culture in 3D! 

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