Product Overview

The major components of LunaGel™ include the ECM proteins collagen type I, III, IV, and V, as well as connective tissue glycoproteins and proteoglycans. LunaGel™ retains the intrinsic cell-instructive bioactivity of natural ECMs facilitating cell attachment, proliferation, differentiation, migration, and proteolytic degradation. LunaGel™’s unique photocrosslinking technology allows unprecedented control over matrix porosity and stiffness, allowing researchers to replicate the physicochemical properties of a variety of healthy and diseased tissues in 3D cell culture applications.

LunaGel™ ECM has been successfully used in a wide range of applications including

• cell attachment and proliferation
• stem cell culture and differentiation
• mechanotransduction assays
• cancer spheroid assays
• angiogenesis assays
• 3D bioprinting
• tissue engineering
• and more

How LunaGel™  Photocrosslinkable Extracellular Matrix​ Works

LunaGel™ is supplied as a sterile solution with freeze-dried photoinitiator. Available as Low Stiffness (0 – 6.5 kPa) and High Stiffness kit (0 – 25 kPa). Reconstitute the photoinitiator in PBS, mix with LunaGel™ ECM and cells, and photocrosslink the solution using the  Luna Crosslinker™ to form 3D cell culture models. By controlling the duration of light exposure you can produce hydrogels with a specified stiffness. Add your favourite culture media and you’re culturing in 3D!

Matrix Stiffness

• Low Stiffness (0 – 6.5 kPa)
• High Stiffness (0 – 25 kPa)

Kit Contents

This kit contains enough LunaGel™ to create a total volume of 10mL (low stiffness) or 7.5mL(high stiffness) hydrogel.

• Low stiffness kit: 5mL LunaGel™ ECM (2 x solution, sterile)
• High stiffness kit: 5mL LunaGel™ ECM (1.5 x solution, sterile)
• 5 vials of photoinitiator (freeze-dried, sterile)

Features

• Liquid at temperatures over 4 °C. No heating or cooling is required.
• Photocrosslinking technology allows highly controlled mechanical properties.
• High Bioactivity (cell attachment, proteolytic degradation).
• Compatible with automated liquid handling.

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